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Global proteomic profiling and identification of stress-responsive proteins using two-dimensional gel electrophoresis

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Title Global proteomic profiling and identification of stress-responsive proteins using two-dimensional gel electrophoresis
 
Creator Barua, Pragya
Gayen, Dipak
Lande, Nilesh Vikram
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Global proteome
Mass spectrometry
Stress-responsive proteins
Two-dimensional gel electrophoresis
 
Description Accepted date: 1 Jan 2017
Global proteome profiling is a direct representation of the protein set in an organism, organ, tissues, or an organelle. One of the main objectives of proteomic analysis is the comparison and relative quantitation of proteins under a defined set of conditions. Two-dimensional gel electrophoresis (2-DE) has gained prominence over the last 4 decades for successfully aiding differential proteomics, providing visual confirmation of changes in protein abundance, which otherwise cannot be predicted from genome analysis. Each protein spot on 2-DE gel can be analyzed by its abundance, location, or even its presence or absence. This versatile gel-based method combines and utilizes the finest principle for separation of protein complexes by virtue of their charge and mass, visual mapping coupled with successful mass spectrometric identification of individual proteins.
 
Date 2017-07-25T05:16:57Z
2017-07-25T05:16:57Z
2017
 
Type Article
 
Identifier Methods in Molecular Biology, 1631: 163-179.
978-1-4939-7136-7
http://59.163.192.83:8080/jspui/handle/123456789/771
https://link.springer.com/protocol/10.1007/978-1-4939-7136-7_10
https://doi.org/10.1007/978-1-4939-7136-7_10
 
Language en_US
 
Publisher Springer