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Transcriptome changes in Arabidopsis thaliana infected with Pseudomonas syringae during drought recovery

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Title Transcriptome changes in Arabidopsis thaliana infected with Pseudomonas syringae during drought recovery
 
Creator Gupta, Aarti
Senthil-Kumar, Muthappa
 
Subject Pseudomonas syringae
Arabidopsis thaliana
 
Description Accepted date: 24 July 2017
Field-grown plants experience cycles of drought stress and recovery due to variation in soil moisture
status. Physiological, biochemical and transcriptome responses instigated by recovery are expected to
be different from drought stress and non-stressed state. Such responses can further aid or antagonize
the plant’s interaction with the pathogen. However, at molecular level, not much is known about plantpathogen
interaction during drought recovery. In the present study, we performed a microarray-based
global transcriptome profiling and demonstrated the existence of unique transcriptional changes in
Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tomato DC3000 at the time of drought
recovery (drought recovery pathogen, DRP) when compared to the individual drought (D) or pathogen
(P) or drought recovery (DR). Furthermore, the comparison of DRP with D or DR and P transcriptome
revealed the presence of a few common genes among three treatments. Notably, a gene encoding
proline dehydrogenase (AtProDH1) was found to be commonly up-regulated under drought recovery
(DR), DRP and P stresses. We also report an up-regulation of pyrroline-5-carboxylate biosynthesis
pathway during recovery. We propose that AtProDH1 influences the defense pathways during DRP.
Altogether, this study provides insight into the understanding of defense responses that operate in
pathogen-infected plants during drought recovery.
Projects at M.S.-K. lab are supported by DBT-Ramalingaswami re-entry fellowship grant (BT/RLF/reentry/23/2012).
A.G. acknowledges SERB National Post-Doctoral Fellowship (N-PDF/2015/000116). NIPGR
Plant Growth Facility is also duly acknowledged. We thank Mr. Siva Shanmugam for performing CytoscapeBiNGO
based GO enrichment. We also thank Mr. Sundar, Mr. Rahim, Mr. Prem Negi and Mr. Ashok Kumar for
extending technical help at the laboratory and central instrumentation facility. Authors thank Mr. Mehanathan
Muthamilarasan and Mr. Joel Lars Fernandez for critical reading of the manuscript.
 
Date 2017-08-14T05:04:54Z
2017-08-14T05:04:54Z
2017
 
Type Article
 
Identifier Scientific Reports, 7: 9124
2045-2322
http://59.163.192.83:8080/jspui/handle/123456789/773
https://www.nature.com/srep/articles?type=article
10.1038/s41598-017-09135-y
 
Language en_US
 
Publisher Nature Publishing Group