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GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana

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Title GBF3 transcription factor imparts drought tolerance in Arabidopsis thaliana
 
Creator Ramegowda, Venkategowda
Gill, Upinder Singh
Sivalingam, Palaiyur Nanjappan
Gupta, Aarti
Gupta, Chirag
Govind, Geetha
Nataraja, Karaba N.
Pereira, Andy
Udayakumar, Makarla
Mysore, Kirankumar S.
Senthil-Kumar, Muthappa
 
Subject Arabidopsis thaliana
GBF3
transcription factor
drought
tolerance
 
Description Accepted date: 24 July 2017
Drought transcriptome analysis of finger millet (Eleusine coracana) by cDNA subtraction identified
drought responsive genes that have a potential role in drought tolerance. Through virus-induced gene
silencing (VIGS) in a related crop species, maize (Zea mays), several genes, including a G-BOX BINDING
FACTOR 3 (GBF3) were identified as candidate drought stress response genes and the role of GBF3 in
drought tolerance was studied in Arabidopsis thaliana. Overexpression of both EcGBF3 and AtGBF3
in A. thaliana resulted in improved tolerance to osmotic stress, salinity and drought stress in addition
to conferring insensitivity to ABA. Conversely, loss of function of this gene increased the sensitivity of
A. thaliana plants to drought stress. EcGBF3 transgenic A. thaliana results also suggest that drought
tolerance of sensitive plants can be improved by transferring genes from far related crops like finger
millet. Our results demonstrate the role of GBF3 in imparting drought tolerance in A. thaliana and
indicate the conserved role of this gene in drought and other abiotic stress tolerance in several plant
species.
Projects at MS-K lab are supported by National Institute of Plant Genome Research core funding and Department
of Biotechnology (BT/RLF/re-entry/23/2012). Projects at KSM lab are supported by Noble Research Institute
and National Science Foundation. Projects at KNN & MU lab are supported by grants from the Department
of Biotechnology (transcription factor project grant number F.No. BT/PR/4946/Agr/16/421/2004) and Indian
council of Agricultural Research - Niche Area of Excellence program (F.No. 10-(6)/2005 EPD) & (F.No.
10(15)2012 EPD). PNS acknowledge funding from Indian Council of Agricultural Research for his visit to the
Noble Research Institute. Authors thank Drs Mehanathan Muthamilarasan and Prachi Pandey for critical reading
of the manuscript. Authors also thank Miss Subnum Gerose Dungdung, UG trainee in MS-K lab for technical
help.
 
Date 2017-08-14T05:17:29Z
2017-08-14T05:17:29Z
2017
 
Type Article
 
Identifier Scientific Reports, 7: 9148
2045-2322
http://59.163.192.83:8080/jspui/handle/123456789/774
https://www.nature.com/srep/articles?type=article
10.1038/s41598-017-09542-1
 
Language en_US
 
Publisher Nature Publishing Group