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General Control Non-repressible-4 (GCN4) degrades 14-3-3 and the RIN4 complex to regulate stomatal aperture with implications on nonhost disease resistance and drought tolerance

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Title General Control Non-repressible-4 (GCN4) degrades 14-3-3 and the RIN4 complex to regulate stomatal aperture with implications on nonhost disease resistance and drought tolerance
 
Creator Kaundal, Amita
Ramu, Vemanna S
Oh, Sunhee
Lee, Seonghee
Pant, Bikram
Lee, Hee-Kyung
Rojas, Clemencia M.
Senthil-Kumar, Muthappa
Mysore, Kirankumar S.
 
Subject stomatal aperture size
GCN4
Drought Tolerance
Nonhost Disease Resistance
General Control Non-repressible-4
 
Description Accepted date: August 28, 2017
Plants have complex and adaptive innate immune responses against pathogen infections. Stomata are key entry points for many plant pathogens. Both pathogens and plants regulate stomatal aperture for pathogen entry and defense, respectively. Not all plant proteins involved in stomatal aperture regulation have been identified. Here we report general control non-repressible-4 (GCN4), an AAA+-ATPase family protein, as one of the key proteins regulating stomatal aperture during biotic and abiotic stress. Silencing of GCN4 in Nicotiana benthamiana and Arabidopsis compromises host and nonhost disease resistance due to open stomata during pathogen infection. AtGCN4 overexpression plants have reduced H+-ATPase activity, less responsive stomata to coronatine or fusicoccin, have reduced pathogen entry, and confers drought tolerance. This study also demonstrates that AtGCN4 interacts with RIN4 and 14-3-3 proteins and suggest that GCN4 may degrade RIN4 and 14-3-3 proteins via a proteasome mediated pathway to reduce the activity of plasma membrane H+-ATPase complex thus reducing proton pump activity to close stomata.
We thank Dr. Gitta Coaker (UC, Davis) for providing RIN4 OE and rin4 mutant seeds and
Janie Gallaway for assistance with plant care. This work was supported by the Noble
Research Institute, LLC. The Leica confocal system used in this study was purchased
using a National Science Foundation grant (DBI 0400580). V.S.R acknowledges
Fulbright-Nehru postdoctoral fellowship from USIEF, INDIA.
 
Date 2017-09-01T11:19:39Z
2017-09-01T11:19:39Z
2017
 
Type Article
 
Identifier The Plant Cell, (In Press)
1531-298X
http://223.31.159.10:8080/jspui/handle/123456789/779
http://www.plantcell.org/content/early/2017/08/30/tpc.17.00070
https://doi.org/10.1105/tpc.17.00070
 
Language en_US
 
Publisher American Society of Plant Biologists