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A prophage tail-like protein is deployed by Burkholderia bacteria to feed on fungi

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Title A prophage tail-like protein is deployed by Burkholderia bacteria to feed on fungi
 
Creator Swain, Durga Madhab
Yadav, Sunil Kumar
Tyagi, Isha
Kumar, Rahul
Kumar, Rajeev
Ghosh, Srayan
Das, Joyati
Jha, Gopaljee
 
Subject Bacterial secretion
Fungi
Microbial ecology
Antifungal agents
Burkholderia
tail-like protein
prophage
 
Description Accepted date: 05 July 2017
Some bacteria can feed on fungi, a phenomenon known as mycophagy. Here we show that a prophage tail-like protein (Bg_9562) is essential for mycophagy in Burkholderia gladioli strain NGJ1. The purified protein causes hyphal disintegration and inhibits growth of several fungal species. Disruption of the Bg_9562 gene abolishes mycophagy. Bg_9562 is a potential effector secreted by a type III secretion system (T3SS) and is translocated into fungal mycelia during confrontation. Heterologous expression of Bg_9562 in another bacterial species, Ralstonia solanacearum, confers mycophagous ability in a T3SS-dependent manner. We propose that the ability to feed on fungi conferred by Bg_9562 may help the bacteria to survive in certain ecological niches. Furthermore, considering its broad-spectrum antifungal activity, the protein may be potentially useful in biotechnological applications to control fungal diseases.
I.T. and S.G. acknowledge fellowship from CSIR, Govt of India, while S.K.Y. and J.D.
acknowledge fellowship from DBT, Govt. of India. We thank R.V. Sonti for providing
E. coli strains, S.K. Ray for providing Ralstonia solanacearum F1C1 and its T3SS deficient
mutant (F1C1N2), N. Tuteja for providing pET28a vector and T. R. Sharma for
Magnaporthe oryzae strain. We sincerely thank R.V. Sonti, S.K. Ray, and Imran Siddiqi
for comments on the manuscript. This work was supported by core research grant from
National Institute of Plant Genome Research, India. Also, the research funding from
DBT, Government of India to support the GJ lab is gratefully acknowledged.
 
Date 2017-09-04T06:44:02Z
2017-09-04T06:44:02Z
2017
 
Type Article
 
Identifier Nature Communications 8(1): 404
2041-1723
http://223.31.159.10:8080/jspui/handle/123456789/782
https://www.nature.com/articles/s41467-017-00529-0
10.1038/s41467-017-00529-0
 
Language en_US
 
Publisher Nature Publishing Group