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Isolation and structural studies of mitochondria from pea roots
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View Archive Info| Field | Value | |
| Title |
Isolation and structural studies of mitochondria from pea roots
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| Creator |
Vishwakarma, Abhaypratap
Gupta, Kapuganti Jagadis |
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| Subject |
Confocal microscope
Mitochondria Pea Percoll gradient |
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| Description |
Accepted date: 5 Sept 2017
For structural and respiratory studies, isolation of intact and active mitochondria is essential. Here, we describe an isolation method which gave good yield and intact mitochondria from 2-week-old pea (Pisum sativum) roots grown hydroponically under standard growth conditions. We used Percoll gradient centrifugation for this isolation procedure. The yield of purified mitochondria was 50 μg/g FW. Isolated mitochondria maintained their structure which was observed by using MitoTracker green in confocal microscope and scanning electron microscopy (SEM). Intact mitochondria are clearly visible in SCM images. Taken together this isolation method can be used for physiological and microscopic studies on mitochondria. |
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| Date |
2017-09-11T10:45:37Z
2017-09-11T10:45:37Z 2017 |
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| Type |
Book chapter
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| Identifier |
Methods in Molecular Biology, 1670: 87-95
978-1-4939-7292-0 http://223.31.159.10:8080/jspui/handle/123456789/785 https://link.springer.com/protocol/10.1007%2F978-1-4939-7292-0_10 https://doi.org/10.1007/978-1-4939-7292-0_10 |
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| Language |
en_US
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| Publisher |
Springer
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