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Isolation and structural studies of mitochondria from pea roots

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Title Isolation and structural studies of mitochondria from pea roots
 
Creator Vishwakarma, Abhaypratap
Gupta, Kapuganti Jagadis
 
Subject Confocal microscope
Mitochondria
Pea
Percoll gradient
 
Description Accepted date: 5 Sept 2017
For structural and respiratory studies, isolation of intact and active mitochondria is essential. Here, we describe an isolation method which gave good yield and intact mitochondria from 2-week-old pea (Pisum sativum) roots grown hydroponically under standard growth conditions. We used Percoll gradient centrifugation for this isolation procedure. The yield of purified mitochondria was 50 μg/g FW. Isolated mitochondria maintained their structure which was observed by using MitoTracker green in confocal microscope and scanning electron microscopy (SEM). Intact mitochondria are clearly visible in SCM images. Taken together this isolation method can be used for physiological and microscopic studies on mitochondria.
 
Date 2017-09-11T10:45:37Z
2017-09-11T10:45:37Z
2017
 
Type Book chapter
 
Identifier Methods in Molecular Biology, 1670: 87-95
978-1-4939-7292-0
http://223.31.159.10:8080/jspui/handle/123456789/785
https://link.springer.com/protocol/10.1007%2F978-1-4939-7292-0_10
https://doi.org/10.1007/978-1-4939-7292-0_10
 
Language en_US
 
Publisher Springer