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Isolation of physiologically active and intact mitochondria from chickpea

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Title Isolation of physiologically active and intact mitochondria from chickpea
 
Creator Pandey, Sonika
Kumari, Aprajita
Gupta, Kapuganti Jagadis
 
Subject Chickpea
Confocal
Germination
Mitochondria
Oxygen sensor
Respiration rate
 
Description Accepted date: 5 Sept 2017
Chickpea is an important leguminous crop that belongs to Fabaceae family, highly valued for its nutritious seeds. Seeds contain reserve food for the developing embryos. Mitochondria are crucial organelle for generation of chemical energy in the form of ATP which is required for achieving metabolically active state; therefore, investigating mitochondrial function and respiration rate is crucial for exploring various metabolic and physio-biochemical changes that occur during seed germination. Here we describe a method for isolation of mitochondria from germinating seeds of two chickpea varieties, i.e., Desi and Kabuli. Structure of Mitotracker-stained isolated mitochondria was observed by confocal microscopy and respiration rate was measured using an oxygen microsensor.
 
Date 2017-09-11T10:54:43Z
2017-09-11T10:54:43Z
2017
 
Type Book chapter
 
Identifier Methods in Molecular Biology, 1670: 77-85
978-1-4939-7292-0
http://223.31.159.10:8080/jspui/handle/123456789/786
https://link.springer.com/protocol/10.1007%2F978-1-4939-7292-0_9
https://doi.org/10.1007/978-1-4939-7292-0_9
 
Language en_US
 
Publisher Springer