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Measurement of oxygen status in Arabidopsis leaves undergoing the hypersensitive response during Pseudomonas infection

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Title Measurement of oxygen status in Arabidopsis leaves undergoing the hypersensitive response during Pseudomonas infection
 
Creator Kumari, Aprajita
Preston, Gail M.
Gupta, Kapuganti Jagadis
 
Subject Arabidopsis
Hypersensitive response
Oxygen
Programmed cell death
VisiSens
 
Description Accepted date: 5 Sept 2017
When plants are infected with pathogens they defend themselves via various processes. Once such process is the development of the hypersensitive response (HR), a kind of programmed cell death (PCD), in which localized cell death takes place in order to prevent pathogen spread to other part of tissue. The Arabidopsis and Pseudomonas syringae system is one of the best known examples to study the HR. Here we used the VisiSens™ oxygen-imaging system to investigate oxygen distributions in Arabidopsis leaves infected with an avirulent strain of P. syringae and undergoing the hypersensitive response. Using this method we observed a change in oxygen status at 6 h post-infection and a drop in oxygen levels at 24 h after infection.
 
Date 2017-09-11T11:22:25Z
2017-09-11T11:22:25Z
2017
 
Type Book chapter
 
Identifier Methods in Molecular Biology, 1670: 71-76
978-1-4939-7292-0
http://223.31.159.10:8080/jspui/handle/123456789/787
https://link.springer.com/protocol/10.1007%2F978-1-4939-7292-0_8
https://doi.org/10.1007/978-1-4939-7292-0_8
 
Language en_US
 
Publisher Springer