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Measurement of respiration and internal oxygen in germinating Cicer arietinum L. seeds using optic microsensor

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Title Measurement of respiration and internal oxygen in germinating Cicer arietinum L. seeds using optic microsensor
 
Creator Pandey, Sonika
Kumari, Aprajita
Bharadwaj, Chellapilla
Gupta, Kapuganti Jagadis
 
Subject Germination
Internal oxygen
Microsensor
Respiration
Seed
 
Description Accepted date: 5 Sept 2017
Internal oxygen concentrations vary in different tissues depending on tissue size, developmental stage, and their location. Respiratory rate of tissue also determines internal oxygen levels. For studying various signaling pathways it is essential to establish a correlation between respiration and internal oxygen. Seed germination is associated with increase in respiration which can dictate the internal oxygen and subsequent production of reactive oxygen species. Using optic oxygen microsensor we made an attempt to measure respiratory rate and internal oxygen. We found that microsensor is able to sense internal oxygen and it is also possible to measure oxygen levels in a close vial that contains seeds. Step-by-step protocol is described here along with illustration.
 
Date 2017-09-11T11:40:32Z
2017-09-11T11:40:32Z
2017
 
Type Book chapter
 
Identifier Methods in Molecular Biology, 1670: 57-62
978-1-4939-7292-0
http://223.31.159.10:8080/jspui/handle/123456789/789
https://link.springer.com/protocol/10.1007%2F978-1-4939-7292-0_6
https://doi.org/10.1007/978-1-4939-7292-0_6
 
Language en_US
 
Publisher Springer