Record Details

Isolation and Confirmation of Listeria Species from Seafood off Goa Region by Polymerase Chain Reaction.

DRS at CSIR-National Institute of Oceanography

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Title Isolation and Confirmation of Listeria Species from Seafood off Goa Region by Polymerase Chain Reaction.
 
Creator Gawade, L.
Barbuddhe, S.B.
Bhosle, S.
 
Subject public health
microbial contamination
polymerization
seafood
Listeria monocytogenes
 
Description Several food borne outbreaks have highlighted the importance of Listeria monocytogenes to the public health and have been recognized as an emerging, important food borne pathogen, and a causative agent of listerioses. A number of genes are involved in the manifestation of Listeria virulence, hlyA is one among them. In the present study, 111 marine fish samples including prawns, finfishes and bivalves were screened for the presence of Listeria species. The isolates were characterized biochemically and further L. monocytogenes were confirmed by polymerase chain reaction (PCR) technique using the hlyA gene as a tool to differentiate between L. monocytogenes and other non-pathogenic Listeria species. Out of 111 samples 5 (4.5%) samples were positive for L. monocytogenes. Among the three different types of samples bivalves were found to have maximum percent (12.5) of L. monocytogenes followed by prawns (3.84) and finfishes (2.9). Among all the 111 samples, 15 (13.51%) samples were positive for other Listeria species. It was observed that Listeria occurrence is more in shellfishes than in fin fishes. All the isolates were sensitive towards five different antibiotics in sequence ciprofloxacin > sulphafurazole > norfloxacin > ampicillin and gentamicin.
 
Date 2017-09-27T13:04:17Z
2017-09-27T13:04:17Z
2010
 
Type Journal Article
 
Identifier Indian Journal of Microbiology, vol.50; 2010; 385-389.
http://drs.nio.org/drs/handle/2264/5143
 
Language en
 
Rights An edited version of this paper was published by Springer. This paper is for R & D purpose and Copyright [2010] Springer.
 
Publisher Springer