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Attenuation of Pseudomonas aeruginosa virulence by marine invertebrate–derived Streptomyces sp.

DRS at CSIR-National Institute of Oceanography

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Title Attenuation of Pseudomonas aeruginosa virulence by marine invertebrate–derived Streptomyces sp.
 
Creator Naik, D.N.
Wahidullah, S.
Meena, R.M.
 
Subject Pseudomonas aeruginosa
Chromobacterium violaceum
Invertebrate biology::Physiology, biochemistry, biophysics
Invertebrate biology::Physiology, biochemistry, biophysics
 
Description The study aimed to discover quorum sensing (QS) inhibitors from marine sponge-derived actinomycetes and analyse its inhibitory activities against QS-mediated virulence factors in Pseudomonas aeruginosa. Seventy-two actinomycetes isolated from marine invertebrates collected from the western coast of India were screened against the QS indicator strain Chromobacterium violaceum CV12472. Methanol extracts of 12 actinomycetes showing inhibition of violacein production were accessed for down-regulation of QS-mediated virulence factors like swarming, biofilm formation, pyocyanin, rhamnolipid and LasA production in Ps. aeruginosa ATCC 27853. The isolates NIO 10068, NIO 10058 and NIO 10090 exhibited very good anti-QS activity, with NIO 10068 being the most promising one. Mass spectrometric analysis of NIO 10068 methanol extract revealed the presence of cinnamic acid and linear dipeptides proline-glycine and N-amido-a-proline in the active extract. Detailed investigation suggested that although linear dipeptide Pro-Gly is to some extent responsible for the observed biological activity, cinnamic acid seems to be the main compound responsible for it. Marine-derived actinomycetes are a potential storehouse for QS inhibitors.
 
Date 2017-09-27T13:05:30Z
2017-09-27T13:05:30Z
2012
 
Type Journal Article
 
Identifier Letters in Applied Microbiology, vol.56; 2012; 197-207.
http://drs.nio.org/drs/handle/2264/5154
 
Language en
 
Rights Copyright [2012]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
 
Publisher John Wiley & Sons