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PCR detection of ansA from marine bacteria and its sequence characteristics from Bacillus tequilensis NIOS4

DRS at CSIR-National Institute of Oceanography

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Title PCR detection of ansA from marine bacteria and its sequence characteristics from Bacillus tequilensis NIOS4
 
Creator Nayak, S.
Porob, S.
Fernandes, Areena
Meena, R.M.
Ramaiah, N.
 
Subject MICROBIOLOGY::Genetics and evolution
MICROBIOLOGY::Physiology, biochemistry, biophysics
GEOLOGY AND GEOPHYSICS::Sediments and sedimentation
 
Description As many as 71 marine bacterial DNA extracts were PCR screened for L-asparaginase (ansA), a key gene in anti-cancer molecular-searches. Over 62% (44) of them were positive for ansA gene. The positive cultures were from genera Bacillus and Staphylococcus. The ansA gene cloned from isolate NIOS4 belonging to recently described Bacillus tequilensis is 1099 bp in length with a 990 bp ORF coding for 329 amino acids. BLASTx analysis revealed this sequence to be 98% similar to earlier reported ansA sequence from B. subtilis (Accession no. NP390239.1). By comparing its deduced amino acid sequence with other bacterial asparaginase sequences six substitutions at positions 305(Thr), 313(Lys), 314(Leu), 315(Asp), 318(Arg), and 320(Gln) are observed. Key residues like Thr(12), Thr(85), Asp(86), Lys(156), and Phe(165) taking part in active-site formation and imparting catalytic properties are conserved. The phylogenetic tree based of the ansA amino acid sequences revealed close relatedness of the NIOS4 ansA sequence with B. subtilis (Accession no. NP 390239.1). It's very close genetic resemblance to B. subtilis and conservation of certain key amino acid residues suggest it as a prospective candidate for evaluation and, production of L-asparaginases
 
Date 2017-09-27T13:06:39Z
2017-09-27T13:06:39Z
2014
 
Type Journal Article
 
Identifier Journal of Basic Microbiology, vol.54(2); 2014; 162-168
http://drs.nio.org/drs/handle/2264/5165
 
Language en
 
Rights Prior to publication, the Contributor must include the following notice on the preprint: “This is a preprint of an article accepted for publication in Journal of Basic Microbiology. Copyright © [2014] WILEY-VCH Verlag GmbH & Co. ”. After publication of the Contribution by Wiley, the preprint notice should be amended to read as follows: “This is a preprint of an article published in [Journal of Basic Microbiology, vol.54(2); 2014; 162-168]”and an electronic link to the Journal’s WWW site, located at the following Wiley URL: http://onlinelibrary.wiley.com/doi/10.1002/jobm.201200355/abstract. The Contributor agrees not to update the preprint or replace it with the published version of the Contribution.
 
Publisher Wiley-VCH Verlag GmbH & Co.