Record Details

Radioprotection against DNA damage by an extract of Indian green mussel, Perna viridis (L.)

DRS at CSIR-National Institute of Oceanography

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Title Radioprotection against DNA damage by an extract of Indian green mussel, Perna viridis (L.)
 
Creator Kumaran, S.P.
Kutty, B.C.
Chatterji, A.
Parameswaran, P.S.
Mishra, K.P.
 
Subject Geology and Geophysics
marine molluscs
DNA
plasmids
biological damage
chemical extraction
Perna viridis
ISW, India, Goa
 
Description This study describes the radioprotective ability of a hydrolysate prepared using an enzymeacid hydrolysis method from the green mussel Perna viridis in terms of its ability to prevent radiation-induced damage in plasmid DNA, cell death, reactive oxygen species (ROS) formation, and DNA damage in mice lymphocytes The mussel hydrolysate (MH) present during irradiation showed significant protection from Gamma-radiation-induced strand breaks in plasmid DNA as evaluated by gel electrophoresis Viability studies by trypan blue dye exclusion and MTT assay showed that pre incubation of mice splenic lymphocytes with MH protected them from Gamma-radiation mediated killing Moreover, the presence of MH during irradiation of isolated mice lymphocytes significantly decreased the DNA damage, as measured by comet assay Measurement of intracellular ROS by dichlorofluorescein fluorescence revealed that the presence of MH effectively reduced the ROS generated in lymphocytes by both chemical method and Gamma-irradiation Prevention of DNA damage both in plasmid and lymphocytes and cell death in lymphocytes appears correlated with reduction of oxidatively generated free radicals It is concluded that protection against radiation-induced cell death and DNA damage by MH was attributable to reduction of reactive free radical species generated by Gamma-radiation
 
Date 2008-08-07T10:20:41Z
2017-09-30T03:07:59Z
2008-08-07T10:20:41Z
2017-09-30T03:07:59Z
2007
 
Type Journal Article
 
Identifier Journal of Environmental Pathology Toxicology and Oncology, vol.26(4); 2007; 263-273
0731-8898
http://drs.nio.org/drs/handle/2264/7583
 
Language en
 
Relation J Environ Pathol Tox
SCI
 
Publisher Begell House