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A comparative study of coastal and clinical isolates of Pseudomonas aeruginosa

DRS at CSIR-National Institute of Oceanography

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Title A comparative study of coastal and clinical isolates of Pseudomonas aeruginosa
 
Creator Nair, A.V.
Joseph, N.
Krishna, K.
Sneha, K.G.
Tom, N.
Jangid, K.
Nair S.
 
Subject Microbiology
Bacteria
Distribution
Fatty acids
Environment effect
ISW, India
 
Description Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium having a versatile metabolic potential and great ecological and clinical significance The geographical distribution of P aeruginosahas revealed the existence of an unbiased genetic arrangement in terrestrial isolates In contrast, there are very few reports about P aeruginosa strains from marine environments The present work was aimed at studying the distribution of P aeruginosa in coastal waters along the Indian Peninsula and understanding the environmental influence on genotypic, metabolic and phenotypic characteristics by comparing marine and clinical isolates Of the 785 marine isolates obtained on selective media, only 32 (~4.1%) were identified as P aeruginosa, based on their fatty acid methyl ester profiles A low Euclidian distance value (< 2.5) obtained from chemotaxonomic analysis suggested that all the environmental (coastal and marine) isolates originated from a single species While UPGMA analyses of AP-PCR and phenotypic profiles separated the environmental and clinical isolates, fatty acid biotyping showed overlapping between most clinical and environmental isolates Our study revealed the genetic diversity among different environmental isolates of P aeruginosa While biogeographical separation was not evident based solely on phenotypic and metabolic typing, genomic and metatranscriptomic studies are more likely to show differences between these isolates Thus, newer and more insightful methods are required to understand the ecological distribution of this complex group of bacteria.
 
Date 2016-05-13T10:59:21Z
2017-09-30T03:12:35Z
2016-05-13T10:59:21Z
2017-09-30T03:12:35Z
2015
 
Type Journal Article
 
Identifier Brazilian Journal of Microbiology, vol.46(3); 2015; 725-734
1517-8382
http://drs.nio.org/drs/handle/2264/7645
 
Language en
 
Relation Braz J Microbiol
SCI
 
Publisher Elsevier