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Calculation of antibody and antigen concentrations from ELISA data using a graphical method.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Calculation of antibody and antigen concentrations from ELISA data using a graphical method.
 
Creator Raghava, G.P.S.
Joshi, A
Agrewala, J N
 
Subject QR180 Immunology
 
Description A graphical method for determining the concentration of either the antibody or the antigen from ELISA data is presented in the form of a GWBASIC program. In the program, ELISAEQ, optical densities (OD) obtained from a 96-well ELISA plate can be input either directly by interfacing a microplate reader to the computer or manually. The program uses standard sample data, and selects the semilogarithmic linear range. Over this range, a least-squares method is used to determine the concentrations of interest. In addition, a hyperbolic interpolation formula is derived over the entire range for estimating the antibody or antigen concentration of the unknown samples whose OD is beyond the linear range.
 
Publisher Elsevier Science
 
Date 1992-08-30
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/114/1/raghava1992.pdf
Raghava, G.P.S. and Joshi, A and Agrewala, J N (1992) Calculation of antibody and antigen concentrations from ELISA data using a graphical method. Journal of immunological methods, 153 (1-2). pp. 263-4. ISSN 0022-1759
 
Relation http://www.sciencedirect.com/science/article/pii/002217599290330V
http://crdd.osdd.net/open/114/