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Differential expression of NF-kappaB in mycobacteria infected THP-1 affects apoptosis.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Differential expression of NF-kappaB in mycobacteria infected THP-1 affects apoptosis.
 
Creator Dhiman, Rohan
Raje, Manoj
Majumdar, Sekhar
 
Subject QD Chemistry
 
Description The present study was conducted to see the role of NF-kappaB in virulent (Mycobacterium tuberculosis H37Rv) and avirulent (M. tuberculosis H37Ra) mycobacterial infection in THP-1 cells. To inactivate NF-kappaB, pCMV-IkappaBalphaM dn containing THP-1 cell line was generated which showed marked increase in apoptosis with M. tuberculosis H37Rv and M. tuberculosis H37Ra. Infected THP-1-IkappaBalphaM dn cells showed decrease in mitochondrial membrane potential, cytochrome c release, activation of caspase-3 and enhanced TNF-alpha production. Increase in apoptosis of infected THP-1-IkappaBalphaM dn cells resulted in inhibition of intracellular mycobacterial growth. Differential NF-kappaB activation potential was observed with M. tuberculosis H37Rv and M. tuberculosis H37Ra. Both the strains activated NF-kappaB after 4 h in THP-1 cells however after 48 h only M. tuberculosis H37Rv activated NF-kappaB which lead to up-regulation of bcl-2 family anti-apoptotic member, bfl-1/A1. Our results indicated that NF-kappaB activation may be a determinant factor for the success of virulent mycobacteria within macrophages.
 
Publisher Elsevier Science
 
Date 2007-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/125/1/majumdar2007.pdf
Dhiman, Rohan and Raje, Manoj and Majumdar, Sekhar (2007) Differential expression of NF-kappaB in mycobacteria infected THP-1 affects apoptosis. Biochimica et biophysica acta, 1770 (4). pp. 649-58. ISSN 0006-3002
 
Relation http://www.sciencedirect.com/science/article/pii/S0304416506003758
http://crdd.osdd.net/open/125/