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Monitoring Arthrobacter protophormiae RKJ100 in a 'tag and chase' method during p-nitrophenol bio-remediation in soil microcosms.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Monitoring Arthrobacter protophormiae RKJ100 in a 'tag and chase' method during p-nitrophenol bio-remediation in soil microcosms.
 
Creator Pandey, Gunjan
Pandey, Janmejay
Jain, R K
 
Subject QR Microbiology
 
Description Monitoring of micro-organisms released deliberately into the environment is essential to assess their movement during the bio-remediation process. During the last few years, DNA-based genetic methods have emerged as the preferred method for such monitoring; however, their use is restricted in cases where organisms used for bio-remediation are not well characterized or where the public domain databases do not provide sufficient information regarding their sequence. For monitoring of such micro-organisms, alternate approaches have to be undertaken. In this study, we have specifically monitored a p-nitrophenol (PNP)-degrading organism, Arthrobacter protophormiae RKJ100, using molecular methods during PNP degradation in soil microcosm. Cells were tagged with a transposon-based foreign DNA sequence prior to their introduction into PNP-contaminated microcosms. Later, this artificially introduced DNA sequence was PCR-amplified to distinguish the bio-augmented organism from the indigenous microflora during PNP bio-remediation.
 
Publisher Springer Science
 
Date 2006-05
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/165/1/jain2006.pdf
Pandey, Gunjan and Pandey, Janmejay and Jain, R K (2006) Monitoring Arthrobacter protophormiae RKJ100 in a 'tag and chase' method during p-nitrophenol bio-remediation in soil microcosms. Applied microbiology and biotechnology, 70 (6). pp. 757-60. ISSN 0175-7598
 
Relation http://www.springerlink.com/content/y71081128qh44428/
http://crdd.osdd.net/open/165/