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Comparison of ITS and IGS1 regions for strain typing of clinical and non-clinical isolates of Pichia anomala.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Comparison of ITS and IGS1 regions for strain typing of clinical and non-clinical isolates of Pichia anomala.
 
Creator Sutar, Rajeshwari
David, J K
Ganesan, K
Ghosh, Anup K
Singhi, Sunit
Chakrabarti, Arunaloke
Bachhawat, Anand K
 
Subject QR Microbiology
 
Description Pichia anomala is an emerging nosocomial pathogen and there is a need for methods that distinguish between different P. anomala strains. In the typing of several clinical as well as non-clinical P. anomala strains, the sequence variation of the internal transcribed spacer (ITS) was found to be inadequate for typing purposes. The intergenic spacer 1 (IGS1) region of the rDNA of several P. anomala strains was therefore investigated in detail. The IGS1 region (which varied from 1213 to 1231 bp in length) was interspersed with repeats and had more variation than the ITS regions. Comparative analysis in cases where analysis by the ITS was ambiguous clearly revealed the IGS1 region to be a more discriminatory tool in the typing of P. anomala strains.
 
Publisher Society for General Microbiology (SGM)
 
Date 2004-02
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/225/1/bachhawat2004.pdf
Sutar, Rajeshwari and David, J K and Ganesan, K and Ghosh, Anup K and Singhi, Sunit and Chakrabarti, Arunaloke and Bachhawat, Anand K (2004) Comparison of ITS and IGS1 regions for strain typing of clinical and non-clinical isolates of Pichia anomala. Journal of medical microbiology, 53 (Pt 2). pp. 119-23. ISSN 0022-2615
 
Relation http://jmm.sgmjournals.org/content/53/2/119.long
http://crdd.osdd.net/open/225/