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Investigations into the polymorphisms at the ECM38 locus of two widely used Saccharomyces cerevisiae S288C strains, YPH499 and BY4742.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Investigations into the polymorphisms at the ECM38 locus of two widely used Saccharomyces cerevisiae S288C strains, YPH499 and BY4742.
 
Creator Kumar, Chitranshu
Sharma, Rakesh
Bachhawat, Anand K
 
Subject QR Microbiology
 
Description The ECM38 gene encodes the gamma-glutamyl transpeptidase enzyme, an enzyme involved in glutathione turnover. The enzyme was found to be present in the S288C strain, BY4742, but absent in another widely used strain congenic to S288C, YPH499. Cloning and sequencing the genes from these yeasts indicated the presence of 11 single nucleotide polymorphisms in the coding region and eight single nucleotide polymorphisms in the promoter region of the ECM38 gene of YPH499 (but none in that of BY4742). One of the SNPs in the ECM38 ORF led to a G --> D conversion in a region conserved in all gamma-GT enzymes and was found to be responsible for the loss of activity in this strain. The presence of gamma-GT activity in other YPH strains led us to trace the origins of the polymorphisms in YPH499. Our results indicated that among the progenitor strains, YPH1 and YPH2, YPH1 carried the polymorphisms seen in YPH499 and also lacked the gamma-GT activity. The implications of these results for the use of these widely used S288C strains and the origin of these single nucleotide polymorphisms are presented.
 
Publisher Wiley
 
Date 2003-07-30
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/240/1/bachhawat2003.1.pdf
Kumar, Chitranshu and Sharma, Rakesh and Bachhawat, Anand K (2003) Investigations into the polymorphisms at the ECM38 locus of two widely used Saccharomyces cerevisiae S288C strains, YPH499 and BY4742. Yeast (Chichester, England), 20 (10). pp. 857-63. ISSN 0749-503X
 
Relation http://onlinelibrary.wiley.com/doi/10.1002/yea.1012/pdf
http://crdd.osdd.net/open/240/