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Manipulation of unfolding-induced protein aggregation by peptides selected for aggregate-binding ability through phage display library screening.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Manipulation of unfolding-induced protein aggregation by peptides selected for aggregate-binding ability through phage display library screening.
 
Creator Kundu, Bishwajit
Shukla, Anshuman
Guptasarma, Purnananda
 
Subject QD Chemistry
 
Description A phage-displayed library of peptides (12-mer) was screened for the ability to bind to thermally aggregated bovine carbonic anhydrase (BCA), with a view toward examining whether peptides possessing this ability might bind to partially structured intermediates on the protein's unfolding pathway and, therefore, constitute useful tools for manipulation of the kinetic partitioning of molecules between the unfolded and aggregated states. Two peptides [N-HPSTMGLRTMHP-C and N-TPSAWKTALVKA-C] were identified and tested. While neither showed thermal aggregation autonomously, both peptides individually elicited remarkable increases in the levels of thermal aggregation of BCA. A possible explanation is that both peptides bind to surfaces on molten BCA that are not directly involved in aggregation. Such binding could slow down interconversions between folded and unfolded states and stabilize aggregation-prone intermediate(s) to make them more prone to aggregation, while failing to achieve any steric prevention of aggregation. The approach has the potential of yielding useful aggregation-aiding/inhibiting agents, and may provide clues to whether amorphous aggregates are "immobilized" forms of folding intermediates.
 
Publisher Elsevier Science
 
Date 2002-03-08
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/275/1/guptasarma2002.pdf
Kundu, Bishwajit and Shukla, Anshuman and Guptasarma, Purnananda (2002) Manipulation of unfolding-induced protein aggregation by peptides selected for aggregate-binding ability through phage display library screening. Biochemical and biophysical research communications, 291 (4). pp. 903-7. ISSN 0006-291X
 
Relation http://www.sciencedirect.com/science/article/pii/S0006291X02965496
http://crdd.osdd.net/open/275/