Record Details


Field	Value

Title	Metal ions modulate the plastic nature of Mycobacterium tuberculosis chaperonin-10.

Creator	Taneja, Bhupesh Mande, S C

Subject	QR Microbiology

Description	Chaperonin-10s possess a highly flexible segment of approximately 10 residues that covers their dome-like structure and closes the central cavity of the chaperonin assembly. The dome loop is believed to contribute to the plasticity of their oligomeric structure. We have exploited the presence of a single tryptophan residue occurring in the dome loop of Mycobacterium tuberculosis chaperonin-10 (cpn-10), and through intrinsic fluorescence measurements show that in the absence of metal ions, the tryptophan is almost fully solvent exposed at neutral pH. The dome loop, however, assumes a closed conformation in the presence of metal ions, or at low pH. These changes are fully reversed in the presence of chelating agents such as EDTA, confirming the role of cations in modulating the metastable states of cpn-10.

Publisher	Oxford University Press

Date	2001-06

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://crdd.osdd.net/open/293/1/mande2001.1.pdf Taneja, Bhupesh and Mande, S C (2001) Metal ions modulate the plastic nature of Mycobacterium tuberculosis chaperonin-10. Protein engineering, 14 (6). pp. 391-5. ISSN 0269-2139

Relation	http://peds.oxfordjournals.org/content/14/6/391.long http://crdd.osdd.net/open/293/

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