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SopE acts as an Rab5-specific nucleotide exchange factor and recruits non-prenylated Rab5 on Salmonella-containing phagosomes to promote fusion with early endosomes.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title SopE acts as an Rab5-specific nucleotide exchange factor and recruits non-prenylated Rab5 on Salmonella-containing phagosomes to promote fusion with early endosomes.
 
Creator Mukherjee, K
Parashuraman, S
Raje, Manoj
Mukhopadhyay, A
 
Subject QD Chemistry
 
Description Rab-GTPase regulates the fusion between two specific vesicles. It is well documented that, for their biological function, Rab proteins need to be prenylated for attachment to the vesicle membrane. In contrast, we showed in the present investigation that SopE, a type III secretory protein of Salmonella, translocates onto Salmonella-containing phagosomes (LSP) and mediates the recruitment of non-prenylated Rab5 (Rab5:DeltaC4) on LSP in GTP form. Simultaneously, SopE present in infected cell cytosol acts as an Rab5-specific exchange factor and converts the inactive Rab-GDP to the GTP form. The non-prenylated Rab5 subsequently promoted efficient fusion of LSP with early endosomes. This is the first demonstration that a prenylation-deficient Rab protein retains biological activity and can promote vesicle fusion, if it is recruited on the membrane by some other method.
 
Publisher ASBMB
 
Date 2001-06-29
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/300/1/raje2001.pdf
Mukherjee, K and Parashuraman, S and Raje, Manoj and Mukhopadhyay, A (2001) SopE acts as an Rab5-specific nucleotide exchange factor and recruits non-prenylated Rab5 on Salmonella-containing phagosomes to promote fusion with early endosomes. The Journal of biological chemistry, 276 (26). pp. 23607-15. ISSN 0021-9258
 
Relation http://www.jbc.org/content/276/26/23607.full.pdf+html
http://crdd.osdd.net/open/300/