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Construction of fission yeast vectors with a novel selection strategy that allows their use in wild-type fission yeasts.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Construction of fission yeast vectors with a novel selection strategy that allows their use in wild-type fission yeasts.
 
Creator Ingavale, S S
Sharma, K G
Bachhawat, Anand K
 
Subject QR Microbiology
 
Description Novel vectors that use the Pichia pastoris INO1 gene as a selectable marker and exploit the natural inositol auxotrophy of the fission yeast are described. These plasmids also contained other features desirable in a plasmid cloning vector. These plasmids were evaluated in other species of Schizosaccharomyces and found to replicate autonomously in another variety of S. pombe, S. pombe var. malidevorans. These plasmids can be used for transformation of any wild-type S. pombe strain without the need for selection by induced auxotrophic mutations, or by selection by drug resistance markers, and should greatly assist genetic and molecular manipulations in these yeasts.
 
Publisher Wiley
 
Date 2000-10
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/306/1/bachhawat2000.1.pdf
Ingavale, S S and Sharma, K G and Bachhawat, Anand K (2000) Construction of fission yeast vectors with a novel selection strategy that allows their use in wild-type fission yeasts. Yeast (Chichester, England), 16 (14). pp. 1345-50. ISSN 0749-503X
 
Relation http://onlinelibrary.wiley.com/doi/10.1002/1097-0061(200010)16:14%3C1345::AID-YEA628%3E3.0.CO;2-9/abstract
http://crdd.osdd.net/open/306/