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Characterization and some reaction-engineering aspects of thermostable extracellular beta-galactosidase from a new Bacillus species.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Characterization and some reaction-engineering aspects of thermostable extracellular beta-galactosidase from a new Bacillus species.
 
Creator Sani, R K
Chakraborti, S
Sobti, R C
Patnaik, P R
Banerjee, U C
 
Subject QR Microbiology
 
Description A new strain of Bacillus sp. was isolated from a hot water spring in India. This strain generated a high activity of extracellular beta-galactosidase at 37 degrees C in shake flasks. The beta-galactosidase activity was found to increase continuously but the production rate was slower than with some other organisms reported in the literature. There were noteworthy differences in the time-domain profiles of bacterial concentration and beta-galactosidase activity when the starting concentration of substrate (glucose) was tripled from 10 g/L. These differences may be explained in terms of the relative rates of enzyme synthesis and its diffusion across the cell wall. The enzyme produced by this organism is more stable than other beta-galactosidases; its half-life is 408 h at 50 degrees C and 94 h at 55 degrees C, while the reported enzymes showed perceptible loss of activity within 2 h.
 
Publisher Springer Science
 
Date 1999
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/307/1/banerjee2000.pdf
Sani, R K and Chakraborti, S and Sobti, R C and Patnaik, P R and Banerjee, U C (1999) Characterization and some reaction-engineering aspects of thermostable extracellular beta-galactosidase from a new Bacillus species. Folia microbiologica, 44 (4). pp. 367-71. ISSN 0015-5632
 
Relation http://www.springerlink.com/content/q63318n8g0652m88/fulltext.pdf
http://crdd.osdd.net/open/307/