Record Details

Evidence that phosphate specific transporter is amplified in a fluoroquinolone resistant Mycobacterium smegmatis.

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Evidence that phosphate specific transporter is amplified in a fluoroquinolone resistant Mycobacterium smegmatis.
 
Creator Bhatt, K
Banerjee, S K
Chakraborti, Pradip K
 
Subject QR Microbiology
 
Description We reported in an earlier study that active efflux of drug has a predominant role in conferring resistance in a laboratory-generated ciprofloxacin-resistant mutant of Mycobacterium smegmatis. This mutant exhibited mRNA level overexpression, as well as chromosomal amplification, of the gene pstB, encoding the putative ATPase subunit of phosphate specific transport (Pst) system. We demonstrate here that this mutant shows enhanced phosphate uptake and that inactivation of pstB in the parental strain results in loss of high affinity phosphate uptake and hypersensitivity to fluoroquinolones. These findings suggest a novel role of the Pst system in active efflux, in addition to its involvement in phosphate transport.
 
Publisher Wiley
 
Date 2000-07
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/316/1/chakraborti2000.pdf
Bhatt, K and Banerjee, S K and Chakraborti, Pradip K (2000) Evidence that phosphate specific transporter is amplified in a fluoroquinolone resistant Mycobacterium smegmatis. European journal of biochemistry / FEBS, 267 (13). pp. 4028-32. ISSN 0014-2956
 
Relation http://onlinelibrary.wiley.com/doi/10.1046/j.1432-1327.2000.01437.x/pdf
http://crdd.osdd.net/open/316/