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Identification of an ABC transporter gene that exhibits mRNA level overexpression in fluoroquinolone-resistant Mycobacterium smegmatis.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Identification of an ABC transporter gene that exhibits mRNA level overexpression in fluoroquinolone-resistant Mycobacterium smegmatis.
 
Creator Banerjee, S K
Misra, P
Bhatt, K
Mande, S C
Chakraborti, Pradip K
 
Subject QR Microbiology
 
Description We describe here the PCR amplification of a DNA fragment (mtp1) from Mycobacterium smegmatis using primers derived from consensus sequences of the ABC family of transporters. The fragment encodes amino acid sequences that exhibited significant homology with different ABC transporters. Amino acid sequence alignment of the full length gene with other transporters identified the ABC protein as the B-subunit of the phosphate specific transporter. Strikingly, a M. smegmatis colony which exhibited a high level of ciprofloxacin resistance showed mRNA level overexpression of mtp1. Thus this is the first report in any prokaryote indicating differential expression of an ABC transporter in a fluoroquinolone resistant colony.
 
Publisher Elsevier Science
 
Date 1998-03-20
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/358/1/chakraborti98.pdf
Banerjee, S K and Misra, P and Bhatt, K and Mande, S C and Chakraborti, Pradip K (1998) Identification of an ABC transporter gene that exhibits mRNA level overexpression in fluoroquinolone-resistant Mycobacterium smegmatis. FEBS letters, 425 (1). pp. 151-6. ISSN 0014-5793
 
Relation http://www.sciencedirect.com/science/article/pii/S0014579398002142
http://crdd.osdd.net/open/358/