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Activating piezoelectric crystal surface by silanization for microgravimetric immunobiosensor application.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Activating piezoelectric crystal surface by silanization for microgravimetric immunobiosensor application.
 
Creator Suri, C Raman
Mishra, G C
 
Subject QR Microbiology
 
Description The development of a microgravimetric immunobiosensor using a piezoelectric quartz crystal as a detector requires a stable and reproducible immobilization method for ligand binding. The method of silanization using 3-aminopropyltriethoxysilane (APTES) has been widely used for activating the carrier surface. In the present study, APTES deposition on a piezoelectric crystal surface was studied under various solvent conditions. A fluorescence method, using fluorescence isothiocyanate as a dye, was demonstrated for the quantification of amino groups on the silanized piezoelectric crystal surface. The optimum binding conditions of APTES deposition on a piezoelectric crystal surface were incorporated for the covalent immobilization of protein on the crystal surface in developing a stable and sensitive microgravimetric immunobiosensor. Determination of immunoglobulin G (IgG) concentration was performed using APTES modified piezoelectric crystals coated with protein G. The resonant frequency shift, resulting from the formation of protein G-IgG complex on the crystal surface, correlated with the concentration of IgG in the range 10 ng/ml to 0.1 mg/ml. The APTES modified, protein G coated crystal were found to be quite stable and did not show a significant loss of sensitivity even after 12 weeks of storage at 4 degrees C in a desiccator.
 
Publisher Elsevier Science
 
Date 1996
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/385/1/suri96.pdf
Suri, C Raman and Mishra, G C (1996) Activating piezoelectric crystal surface by silanization for microgravimetric immunobiosensor application. Biosensors & bioelectronics, 11 (12). pp. 1199-205. ISSN 0956-5663
 
Relation http://crdd.osdd.net/open/385/