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Characterization of phage phi O139, a Vibrio cholerae O139 temperate bacteriophage with cohesive DNA termini.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Characterization of phage phi O139, a Vibrio cholerae O139 temperate bacteriophage with cohesive DNA termini.
 
Creator Pajni, S
Chowdhury, N R
Ghosh, A
Kar, S
Ghosh, R K
 
Subject QR Microbiology
 
Description A temperate bacteriophage isolated from Vibrio cholerae O139, the new epidemic strain of cholera, was found to have a polyhedral head 65 nm in diameter and a rigid contractile tail 120 nm in length. The phage chromosome was a double-stranded DNA of 35 kb, with unique cohesive ends and had a G + C content of 58.8%. A restriction map of the phage DNA was constructed using the restriction endonucleases AvaI and BstEII. The phage, whose presence could be detected in nine out of 13 V. cholerae O139 isolates tested, was found to have identical chromosomal integration sites in all the strains. The phage attachment site (attP) was found to be located very close to one end of the genome.
 
Publisher Wiley
 
Date 1995-08-15
 
Type Article
PeerReviewed
 
Relation http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1995.tb07756.x/abstract
http://crdd.osdd.net/open/393/
 
Identifier Pajni, S and Chowdhury, N R and Ghosh, A and Kar, S and Ghosh, R K (1995) Characterization of phage phi O139, a Vibrio cholerae O139 temperate bacteriophage with cohesive DNA termini. FEMS microbiology letters, 131 (1). pp. 69-74. ISSN 0378-1097