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Effect of growth environment on the stability of a recombinant shuttle plasmid, pCPPS-31, in Escherichia coli.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Effect of growth environment on the stability of a recombinant shuttle plasmid, pCPPS-31, in Escherichia coli.
 
Creator Gupta, R
Sharma, P
Vyas, V V
 
Subject QR Microbiology
 
Description The effect of environmental growth conditions on the segregational stability of a recombinant Escherichia coli-Bacillus subtilis shuttle plasmid pCPPS-31 which expresses carboxymethyl cellulase (CMCase) and contains the neomycin resistance gene (Ner), was studied in E. coli DH5 alpha. The stability increased with the decrease in medium complexity. Lowering culture temperatures had a negative effect on stability. Stability was maximum at 100 rpm and a medium/flask volume ratio of 1:10. A pH range of 5-8 had no significant effect on stability. The recombination (rec) and gyrase (gyr) backgrounds of E. coli hosts had no apparent effect on plasmid stability. The plasmid was structurally stable under all circumstances.
 
Publisher Elsevier Science
 
Date 1995-07-15
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/394/1/vyas95.pdf
Gupta, R and Sharma, P and Vyas, V V (1995) Effect of growth environment on the stability of a recombinant shuttle plasmid, pCPPS-31, in Escherichia coli. Journal of biotechnology, 41 (1). pp. 29-37. ISSN 0168-1656
 
Relation http://www.sciencedirect.com/science/article/pii/016816569500049V
http://crdd.osdd.net/open/394/