Record Details

Improved estimation of DNA fragment length from gel electrophoresis data using a graphical method.

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Improved estimation of DNA fragment length from gel electrophoresis data using a graphical method.
 
Creator Raghava, G.P.S.
 
Subject QR Microbiology
 
Description A computer program has been developed for computing DNA fragment size from its electrophoretic mobility using a graphical method. The program uses DNA marker data and selects the semilogarithmic linear range (sl-range), i.e., the linear portion of the semilogarithmic curve (mobility vs. log of DNA fragment length). Over this range a linear interpolation is derived for calculating the size of a DNA fragment whose mobility falls in the sl-range. The program also derives a hyperbolic interpolation formula that covers the entire range for determining the size of a DNA fragment whose mobility is beyond the semilogarithmic linear range. The method described in this paper is sensitive, accurate and reliable. This program can also be used to compute protein or polypeptide size from sodium dodecyl sulfate polyacrylamide gel electrophoresis data. The DOS version of the DNASIZE program is freely available from Netserver at EMBL or from BioTechNet by EMail.
 
Publisher Informa healthcare
 
Date 1994-07
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/408/1/raghava94.pdf
Raghava, G.P.S. (1994) Improved estimation of DNA fragment length from gel electrophoresis data using a graphical method. BioTechniques, 17 (1). pp. 100-4. ISSN 0736-6205
 
Relation http://www.biotechniques.com/BiotechniquesJournal/1994/July/
http://crdd.osdd.net/open/408/