Record Details

Characterization of soluble rifamycin oxidase from Curvularia lunata var. aeria.

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Characterization of soluble rifamycin oxidase from Curvularia lunata var. aeria.
 
Creator Banerjee, U C
 
Subject QR Microbiology
 
Description Curvularia lunata var. aeria was grown on yeast extract, peptone and carboxymethylcellulose (YPC) medium for the production of extracellular rifamycin oxidase. The enzyme was partially purified through a Sephadex G-75 column. The half lives of rifamycin oxidase at 30 degrees and 40 degrees C were 9 d and 100 min, respectively. The activation and deactivation energies of the partially purified enzyme, calculated from Arrhenius plots, were 5.80 and 35.10 kcal mol-1, respectively. The enzyme exhibited a Km (rifamycin B) value of 0.67 mmol l-1 and a Vmax of 11 mumol h-1.ml. Three metal ions, Fe2+, Ag+ and Hg2+, inhibited the enzyme in the 10-20 mmol l-1 metal ion concentration range. Catalytic activity was not affected by the chelating agent, EDTA.
 
Publisher Wiley/Blackwell Scientific Publications
 
Date 1993-07
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/418/1/Banerjee1993%28S%29.pdf
Banerjee, U C (1993) Characterization of soluble rifamycin oxidase from Curvularia lunata var. aeria. Letters in applied microbiology, 17 (1). pp. 1-3. ISSN 0266-8254
 
Relation http://onlinelibrary.wiley.com/doi/10.1111/lam.1993.17.issue-1/issuetoc
http://crdd.osdd.net/open/418/