Record Details

Serine protease inhibitor mediated peptide bond re-synthesis in diverse protein molecules.

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Serine protease inhibitor mediated peptide bond re-synthesis in diverse protein molecules.
 
Creator Sharma, Amit
Radha Kishan, K V
 
Subject QR Microbiology
 
Description Protease inhibitors have been extensively used in research to prevent unwanted degradation of proteins during purification and analysis. Here, we report a remarkable discovery of protease inhibitor mediated reformation of peptide bonds by the serine protease inhibitor, PMSF in a diverse set of proteolyzed molecules. Interestingly, the religation reaction in the presence of PMSF occurs in a very short time period and with very high yields of the religated product. We also investigate the plausible mechanism of such a reaction and demonstrate through biochemical studies and X-ray crystallography that proximity of reacting termini is essential for the feasibility of this reaction.
 
Publisher Elsevier Science
 
Date 2011-11-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/445/1/radha11.pdf
Sharma, Amit and Radha Kishan, K V (2011) Serine protease inhibitor mediated peptide bond re-synthesis in diverse protein molecules. FEBS letters, 585 (21). pp. 3465-70. ISSN 1873-3468
 
Relation http://www.sciencedirect.com/science/article/pii/S0014579311007423
http://crdd.osdd.net/open/445/