Record Details

Pathway for degradation of 2-chloro-4-nitrophenol in Arthrobacter sp. SJCon.

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Pathway for degradation of 2-chloro-4-nitrophenol in Arthrobacter sp. SJCon.
 
Creator Arora, Pankaj Kumar
Jain, R K
 
Subject QR Microbiology
 
Description Degradation of 2-Chloro-4-nitrophenol (2C4NP) was studied by Arthrobacter sp. SJCon, isolated from the soil of a pesticide contaminated site. This strain utilized 2C4NP as sole source of carbon and energy and degraded 2C4NP with stoichiometric release of nitrite and chloride ions. A metabolite was detected during the study of 2C4NP degradation and identified as chlorohydroquinone (CHQ) by thin layer chromatography (TLC), high performance liquid chromatography (HPLC), and gas chromatography-mass spectrometry (GC-MS). Inhibition study using 2,2'-dipyridyl showed that CHQ is a terminal aromatic compound in degradation pathway of 2C4NP. CHQ dioxygenase activity was observed in the crude extract of 2C4NP induced cells of the strain SJCon that suggested the cleavage of the CHQ to maleylacetate (MA). Our study clearly showed that Arthrobacter sp. SJCon degraded 2C4NP via formation of CHQ that further cleaved to MA by CHQ dioxygenase. This mechanism of degradation of 2C4NP differs from previously reported degradation pathways of 2C4NP.
 
Publisher Springer Science
 
Date 2011-12
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/447/1/arora11.1.pdf
Arora, Pankaj Kumar and Jain, R K (2011) Pathway for degradation of 2-chloro-4-nitrophenol in Arthrobacter sp. SJCon. Current microbiology, 63 (6). pp. 568-73. ISSN 1432-0991
 
Relation http://www.springerlink.com/content/023k277u68786137/fulltext.pdf
http://crdd.osdd.net/open/447/