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A novel disposable electrochemical immunosensor for phenyl urea herbicide diuron.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title A novel disposable electrochemical immunosensor for phenyl urea herbicide diuron.
 
Creator Sharma, Priyanka
Sablok, Kavita
Bhalla, Vijayender
Suri, C Raman
 
Subject QD Chemistry
 
Description A disposable electrochemical immunosensor has been developed for the determination of phenyl urea herbicide-diuron using a low cost laser ablated gold electrodes (LC-LAGE) fabricated on polystyrene substrate. The electrodes were electrochemically deposited with prussian blue-gold nanoparticle (PB-GNP) film, and a competitive inhibition immunoassay was performed on LC-LAGE by using a specific hapten-protein conjugate. The binding of available diuron specific antibody on conjugate coated electrode was detected using alkaline phosphatase rabbit anti-IgG antibody. The addition of 1-naphthyl phosphate substrate resulted in the production of electrochemically active product, 1-naphthol, which was monitored using square wave voltammetry technique. The assay exhibited an excellent sensitivity and specificity showing the dynamic response range between 1 ppt and 10 ppm for diuron with detection limit around 1 ppt. This study provides insight into development of a rapid and high-throughput screening of pesticides in environmental samples at a very low cost.
 
Publisher Elsevier Science
 
Date 2011-06-15
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/470/1/suri11.1.pdf
Sharma, Priyanka and Sablok, Kavita and Bhalla, Vijayender and Suri, C Raman (2011) A novel disposable electrochemical immunosensor for phenyl urea herbicide diuron. Biosensors & bioelectronics, 26 (10). pp. 4209-12. ISSN 1873-4235
 
Relation http://www.sciencedirect.com/science/article/pii/S0956566311001758
http://crdd.osdd.net/open/470/