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Novel flavohemoglobins of mycobacteria.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Novel flavohemoglobins of mycobacteria.
 
Creator Gupta, Sanjay
Pawaria, Sudesh
Lu, Changyuan
Yeh, Syun-Ru
Dikshit, Kanak L
 
Subject QR Microbiology
 
Description Flavohemoglobins (flavoHbs) constitute a distinct class of chimeric hemoglobins in which a globin domain is coupled with a ferredoxin reductase such as FAD- and NADH-binding modules. Structural features and active site of heme and reductase domains are highly conserved in various flavoHbs. A new class of flavoHbs, displaying crucial differences in functionally conserved regions of heme and reductase domains, have been identified in mycobacteria. Mining of microbial genome data indicated that the occurrence of such flavoHbs might be restricted to a small group of microbes unlike conventional flavoHbs that are widespread among prokaryotes and lower eukaryotes. One of the representative flavoHbs of this class, encoded by Rv0385 gene (MtbFHb) of Mycobacterium tuberculosis, has been cloned, expressed, and characterized. The ferric and deoxy spectra of MtbFHb displayed a hexacoordinate state indicating that its distal site may be occupied by an intrinsic amino acid or an external ligand and it may not be involved in nitric oxide detoxification. Phylogenetic analysis revealed that mycobacterial flavoHbs constitute a separate cluster distinct from conventional flavoHbs and may have novel function(s).
 
Publisher Wiley
 
Date 2011-05
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/473/1/dikshit11.pdf
Gupta, Sanjay and Pawaria, Sudesh and Lu, Changyuan and Yeh, Syun-Ru and Dikshit, Kanak L (2011) Novel flavohemoglobins of mycobacteria. IUBMB life, 63 (5). pp. 337-45. ISSN 1521-6551
 
Relation http://onlinelibrary.wiley.com/doi/10.1002/iub.460/full
http://crdd.osdd.net/open/473/