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Biotransformation and biomonitoring of phenylurea herbicide diuron.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Biotransformation and biomonitoring of phenylurea herbicide diuron.
 
Creator Sharma, Priyanka
Suri, C Raman
 
Subject QR Microbiology
 
Description A Gram-positive, Micrococcus sp. strain PS-1 isolated from diuron storage site was studied for its capability of biotransformation of phenylurea herbicide diuron to a secondary metabolite, 1-(3,4-dichlorophenyl)urea (DCPU) for bioconjugation and antibody development applications. The metabolite formed associated with profound changes in bacterial cell morphology demonstrated increase in the degradation kinetics of diuron in presence of small quantity of a surfactant. The synthesized metabolite identified by chromatographic and mass spectrometry techniques was conjugated with carrier protein, and used as an immunogen for antibodies production. The generated antibody was highly specific, demonstrating excellent sensitivity against diuron. The antibody was used as receptor molecules in standard fluorescence immunoassay (FIA) format showing detection limit of 0.01 ng/mL in the optimum working concentration range of diuron with good signal precision (∼2%). The study presented first time the degradation pathway of herbicide by specific microorganism to synthesize hapten for bioconjugation and immunoassay development.
 
Publisher Elsevier Science
 
Date 2011-02
 
Type Article
PeerReviewed
 
Relation http://www.sciencedirect.com/science/article/pii/S0960852410017323
http://crdd.osdd.net/open/489/
 
Identifier Sharma, Priyanka and Suri, C Raman (2011) Biotransformation and biomonitoring of phenylurea herbicide diuron. Bioresource technology, 102 (3). pp. 3119-25. ISSN 1873-2976