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Efficient ligation and cloning of DNA fragments with 2-bp overhangs.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Efficient ligation and cloning of DNA fragments with 2-bp overhangs.
 
Creator Ranjan, Rajiv Kumar
Rajagopal, Kammara
 
Subject QD Chemistry
 
Description Various methods of ligation are currently available and routinely used by molecular biologists, such as blunt end ligation, cohesive end (two and four overhangs), and ligation of Taq polymerase-derived products. However, there is no efficient method for the cloning of DNA fragments with 2-bp overhangs. We present a simple method for the efficient ligation of DNA fragments with 2-bp overhanging ends, ranging in size from 0.7 to 2.5 kbp. Our method involves the initial heating and flash freezing of the vector-insert DNA mix, and a subsequent unique ligation reaction. This method provides a new molecular biology tool for researchers.
 
Publisher Elsevier Science
 
Date 2010-07-01
 
Type Article
PeerReviewed
 
Relation http://www.sciencedirect.com/science/article/pii/S0003269710001880
http://crdd.osdd.net/open/526/
 
Identifier Ranjan, Rajiv Kumar and Rajagopal, Kammara (2010) Efficient ligation and cloning of DNA fragments with 2-bp overhangs. Analytical biochemistry, 402 (1). pp. 91-2. ISSN 1096-0309