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Salt-assisted religation of proteolyzed Glutathione-S-transferase follows Hofmeister series.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Salt-assisted religation of proteolyzed Glutathione-S-transferase follows Hofmeister series.
 
Creator Kishan, K V Radha
Sharma, Amit
 
Subject QR Microbiology
 
Description Proteases have been used not only for proteolysis but also in organic solvent-assisted religation processes. Here, we demonstrated the effect of salts on peptide bond resynthesis in Glutathione-S-transferase (GST) and have found it to be in the purview of the Hofmeister phenomena. Our results show that the efficiency and ease of religation increases with an increase in the surface charge densities of the cations used in the study. Thus, the yield of religated GST follows the order: Mg2(+)>Li(+)>Na(+)>K(+). Characteristics of the salt-religated GST were studied using size exclusion chromatography, CD spectroscopy, mass spectrometry and CDNB activity assay. Results show that the properties of salt-religated GST are in close agreement with those of the native GST. Additionally, we also assessed the specific activity of the protease, Subtilisin Carlsberg, used in this study. Contrary, to aqueous-organic systems, wherein there is a remarkable decrease in the proteolytic activity, the activity in the presence of salts is only minimally changed. Our studies suggest that salt-assisted peptide bond formation is favoured primarily due to changes in the ionic environment of the nicked termini of GST, and that there is no role played by the protease.
 
Publisher Bentham Science
 
Date 2010-01
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/554/1/radha10.pdf
Kishan, K V Radha and Sharma, Amit (2010) Salt-assisted religation of proteolyzed Glutathione-S-transferase follows Hofmeister series. Protein and peptide letters, 17 (1). pp. 54-63. ISSN 1875-5305
 
Relation http://www.benthamscience.com/ppl/sample/ppl17-1/0007E.pdf
http://crdd.osdd.net/open/554/