Record Details

Total RNA isolation from recalcitrant yeast cells.

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Total RNA isolation from recalcitrant yeast cells.
 
Creator Amin-ul Mannan, M
Sharma, Sushma
Ganesan, K
 
Subject QD Chemistry
 
Description Conventional methods of RNA isolation are not suitable for yeast cells from stationary phase and fermentation broth. Methods specially reported for such cells are cumbersome and do not lend themselves for use with large number of samples. Here we report a facile method of RNA isolation from such recalcitrant yeast cells. The entire procedure is performed in microcentrifuge tubes and, thus, is ideal for faster processing of multiple samples. The method consistently gives high quality and quantity of RNA, which was found to be suitable for downstream applications such as quantitative real-time polymerase chain reaction. Besides Saccharomyces cerevisiae, the method was found to work equally well with other yeast species; thus, it is likely to have wider applicability.
 
Publisher Elsevier Science
 
Date 2009-06-01
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/565/1/ganesan09.pdf
Amin-ul Mannan, M and Sharma, Sushma and Ganesan, K (2009) Total RNA isolation from recalcitrant yeast cells. Analytical biochemistry, 389 (1). pp. 77-9. ISSN 1096-0309
 
Relation http://www.sciencedirect.com/science/article/pii/S0003269709001742
http://crdd.osdd.net/open/565/