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RT-PCR method for selective detection of silent gene transcripts in silencing mutants in homothallic strains of Schizosaccharomyces pombe.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title RT-PCR method for selective detection of silent gene transcripts in silencing mutants in homothallic strains of Schizosaccharomyces pombe.
 
Creator Ahmed, Shakil
Singh, Jagmohan
 
Subject QH301 Biology
 
Description Here we describe a method that allows selective detection of silent copy transcripts in homothallic strains of Schizosaccharomyces pombe in the presence of the active cassettes. The method involving RT-PCR (reverse transcriptase polymerase chain reaction) exploits our observation that the silent copy transcripts extend beyond the regions of homology to the flanking sequences specific for the donor cassettes, thus allowing design of oligos that are specific for the different donors. The results are validated using a known silencing mutant swi6.
 
Publisher Informa BioSciences
 
Date 2008-01
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/616/1/jagmohan08.pdf
Ahmed, Shakil and Singh, Jagmohan (2008) RT-PCR method for selective detection of silent gene transcripts in silencing mutants in homothallic strains of Schizosaccharomyces pombe. BioTechniques, 44 (1). 54, 56, 58. ISSN 0736-6205
 
Relation http://www.biotechniques.com/multimedia/archive/00003/BTN_A_000112671_O_3569a.pdf
http://crdd.osdd.net/open/616/