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Direct hapten coated immunoassay format for the detection of atrazine and 2,4-dichlorophenoxyacetic acid herbicides.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Direct hapten coated immunoassay format for the detection of atrazine and 2,4-dichlorophenoxyacetic acid herbicides.
 
Creator Kaur, Jasdeep
Boro, Robin C
Wangoo, Nishima
Singh, Kumar Rajesh
Suri, C Raman
 
Subject QD Chemistry
 
Description A novel immunoassay format employing direct coating of small molecular hapten on microtiter plates is reported for the detection of atrazine and 2,4-dichlorophenoxyacetic (2,4-D). In this assay, the polystyrene surface of microtiter plates was first treated with an acid to generate -NO2 groups on the surface. Acid treated plates were further treated with 3-aminoprpyltriethoxysilane (APTES) to functionalize the plate surface with amino groups for covalent linkage to small molecular hapten with carboxyl groups. The modified plates showed significantly high antibody binding in comparison to plates coated with hapten-carrier protein conjugates and presented excellent stability as a function of the buffer pH and reaction time. The developed assay employing direct hapten coated plates and using affinity purified atrazine and 2,4-D antibodies demonstrated very high sensitivity, IC50 values for atrazine and 2,4-D equal to 0.8 ng mL(-1) and 7 ng mL(-1), respectively. The assay could detect atrazine and 2,4-D levels in standard water samples even at a very low concentration upto 0.02 and 0.7 ng mL(-1) respectively in the optimum working range between 0.01 and 1000 ng mL(-1) with good signal reproducibility (p values: 0.091 and 0.224 for atrazine and 2,4-D, respectively). The developed immunoassay format could be used as convenient quantitative tool for the sensitive screening of pesticides in samples.
 
Publisher Elsevier Science
 
Date 2008-01-21
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/619/1/suri08.pdf
Kaur, Jasdeep and Boro, Robin C and Wangoo, Nishima and Singh, Kumar Rajesh and Suri, C Raman (2008) Direct hapten coated immunoassay format for the detection of atrazine and 2,4-dichlorophenoxyacetic acid herbicides. Analytica chimica acta, 607 (1). pp. 92-9. ISSN 1873-4324
 
Relation http://www.sciencedirect.com/science/article/pii/S0003267007019010
http://crdd.osdd.net/open/619/