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Maintenance and stability of introduced genotypes in groundwater aquifer material.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Maintenance and stability of introduced genotypes in groundwater aquifer material.
 
Creator Jain, R K
Sayler, G S
Wilson, J T
Houston, L
Pacia, D
 
Subject QR Microbiology
 
Description Three indigenous groundwater bacterial strains and Pseudomonas putida harboring plasmids TOL (pWWO) and RK2 were introduced into experimentally contaminated groundwater aquifer microcosms. Maintenance of the introduced genotypes was measured over time by colony hybridization with gene probes of various specificity. On the basis of the results of colony hybridization quantitation of the introduced organisms and genes, all introduced genotypes were stably maintained at approximately 10(5) positive hybrid colonies g-1 of aquifer microcosm material throughout an 8-week incubation period. Concomitant removal of the environmental contaminants, viz., toluene, chlorobenzene, and styrene, in both natural (uninoculated) and inoculated aquifer microcosms was also demonstrated. The results indicate that introduced catabolic plasmids, as well as indigenous organisms, can be stably maintained in groundwater aquifer material without specific selective pressure for the introduced genotypes. These results have positive implications for in situ treatment and biodegradation in contaminated aerobic groundwater aquifers.
 
Publisher ASM
 
Date 1987-05
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/642/1/jain1987.pdf
Jain, R K and Sayler, G S and Wilson, J T and Houston, L and Pacia, D (1987) Maintenance and stability of introduced genotypes in groundwater aquifer material. Applied and environmental microbiology, 53 (5). pp. 996-1002. ISSN 0099-2240
 
Relation http://aem.asm.org/content/53/5/996.full.pdf+html
http://crdd.osdd.net/open/642/