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Sensitivity Of Recombinant Fermentation With Run-Away Plasmids: A Structured Analysis Of The Effect Of Dilution Rate.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Sensitivity Of Recombinant Fermentation With Run-Away Plasmids: A Structured Analysis Of The Effect Of Dilution Rate.
 
Creator PATNAIK, P.R.
 
Subject QD Chemistry
 
Description A recently validated four-compartment model is used to describe intra-cellular dynamics during fermentations with recombinant microorganisms containing temperature-sensitive (run-away) plasmids. The time-dependent sensitivities with respect to the dilution rate have been computed for normal and run-away replication. During normal replication the cells are most sensitive in the early stage of fermentation, and the intra-cellular compartment containing RNA and ribosomes is more sensitive than the other three lumped components. This is explained through a buffering effect. During run-away replication the sensitives are either constant or increase slowly with time, and there is a shift in the relative sensitivities of the intra-cellular components. All sensitivities except that of the substrate increase as the dilution rate increases.
 
Publisher Taylor and francis group
 
Date 1995
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/699/1/pat95%28s%29.pdf
PATNAIK, P.R. (1995) Sensitivity Of Recombinant Fermentation With Run-Away Plasmids: A Structured Analysis Of The Effect Of Dilution Rate. Chemical Engineering Communications, 131 (1). pp. 125-140. ISSN 0098-6445
 
Relation http://dx.doi.org/10.1080/00986449508936287
http://crdd.osdd.net/open/699/