Biodegradation of p-nitrophenol via 1,2,4-benzenetriol by an Arthrobacter sp.
DIR@IMTECH: CSIR-Institute of Microbial Technology
View Archive InfoField | Value | |
Title |
Biodegradation of p-nitrophenol via 1,2,4-benzenetriol by an Arthrobacter sp.
|
|
Creator |
Jain, R K
Dreisbach, J H Spain, J C |
|
Subject |
QR Microbiology
|
|
Description |
The degradation of p-nitrophenol (PNP) by Moraxella and Pseudomonas spp. involves an initial monooxygenase-catalyzed removal of the nitro group. The resultant hydroquinone is subject to ring fission catalyzed by a dioxygenase enzyme. We have isolated a strain of an Arthrobacter sp., JS443, capable of degrading PNP with stoichiometric release of nitrite. During induction of the enzymes required for growth on PNP, 1,2,4-benzenetriol was identified as an intermediate by gas chromatography-mass spectroscopy (GC-MS) and radiotracer studies. 1,2,4-Benzenetriol was converted to maleylacetic acid, which was further degraded by the beta-ketoadipate pathway. Conversion of PNP to 1,2,4-benzenetriol is catalyzed by a monooxygenase system in strain JS443 through the formation of 4-nitrocatechol, 4-nitroresorcinol, or both. Our results clearly indicate the existence of an alternative pathway for the biodegradation of PNP.
|
|
Publisher |
ASM
|
|
Date |
1994-08
|
|
Type |
Article
PeerReviewed |
|
Format |
application/pdf
|
|
Identifier |
http://crdd.osdd.net/open/709/1/jain1994.pdf
Jain, R K and Dreisbach, J H and Spain, J C (1994) Biodegradation of p-nitrophenol via 1,2,4-benzenetriol by an Arthrobacter sp. Applied and environmental microbiology, 60 (8). pp. 3030-2. ISSN 0099-2240 |
|
Relation |
http://aem.asm.org/content/60/8/3030.abstract
http://crdd.osdd.net/open/709/ |
|