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Incomplete mixing in large bioreactors ? a study of its role in the fermentative production of streptokinase

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Incomplete mixing in large bioreactors ? a study of its role in the fermentative production of streptokinase
 
Creator Patnaik, P R
 
Subject QD Chemistry
 
Description The production of streptokinase in a batch fermentation has been analysed for the role of incomplete macromixing of the broth. The analysis is based on a kinetic model exhibiting inhibition by the substrate and a primary metabolite (lactic acid), and a mixing model comprising two continuous flow reactors (CFRs) with closed-loop recycle. The inoculum is introduced into one region (one CFR) and the mixing process determines its distribution, growth and reactivity. By varying the dilution rates of the CFRs, any degree of macromixing can be simulated. For dilution rates larger than 1.0 h–1 almost complete macromixing is achieved, for which an analogy has been drawn with micromixing. Increasing the volume of the inoculated region relative to the noninoculated region improves the maximum attainable activity of streptokinase and shortens the time for this. In such a situation an imperfectly mixed bioreactor is superior to a perfectly mixed one, implying that good productivity requires a large inoculated region and incomplete macromixing. These inferences are supported by earlier studies of fluid mixing and relaxation times in bioreactors.
 
Publisher Springer Science
 
Date 1996
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/739/1/pat96%28s2%29.pdf
Patnaik, P R (1996) Incomplete mixing in large bioreactors ? a study of its role in the fermentative production of streptokinase. Bioprocess Engineering, 14 (2). pp. 91-96. ISSN 0178-515X
 
Relation http://dx.doi.org/10.1007/BF00387962
http://crdd.osdd.net/open/739/