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Molecular Characterization ofVibrio choleraeO1 Biotype El Tor Strains Isolated between 1992 and 1995 in Calcutta, India: Evidence for the Emergence of a New Clone of the El Tor Biotype

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Molecular Characterization ofVibrio choleraeO1 Biotype El Tor Strains Isolated between 1992 and 1995 in Calcutta, India: Evidence for the Emergence of a New Clone of the El Tor Biotype
 
Creator Sharma, C
Nair, G B
Mukhopadhyay, A K
Bhattacharya, S K
Ghosh, R K
Ghosh, Amit
 
Subject R Medicine (General)
 
Description Sixty-one clinical strains of Vibrio cholerae Ol El Tor isolated in Calcutta before, during, and after the V. cholerae O139 Bengal outbreak were examined to see if the O1 strains of the post-O139 period were different from those in existence before. Comparison of the restriction fragment length polymorphism of the rRNA genes (ribotyping) and the CTX genetic element revealed that all “before” strains except 1 belonged to a single known ribotype, whereas all “after” strains except 2 belonged to a hitherto undescribed ribotype. Also, 23 of 25 “before” strains harbored two or more copies of CTX in tandem and also a “free” RS1 element away from CTX, whereas 19 of 21 “after” strains had a single copy of CTX and no free RS1 element. CTX occupied different chromosomal locations in “before” and “after” strains. These studies clearly showed that El Tor O1 strains, which displaced V. cholerae O139 in Calcutta, belonged to a new clone and suggested that there is a continuous genetic reassortment among El Tor strains of V. cholerae O1.
 
Publisher Oxford University Press
 
Date 1997
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/752/1/charu1997.pdf
Sharma, C and Nair, G B and Mukhopadhyay, A K and Bhattacharya, S K and Ghosh, R K and Ghosh, Amit (1997) Molecular Characterization ofVibrio choleraeO1 Biotype El Tor Strains Isolated between 1992 and 1995 in Calcutta, India: Evidence for the Emergence of a New Clone of the El Tor Biotype. The Journal of Infectious Diseases, 175 (5). pp. 1134-1141. ISSN 0022-1899
 
Relation http://dx.doi.org/10.1086/516453
http://crdd.osdd.net/open/752/