ICAR Research Data Repository for Knowledge Management
Strain improvement for the production of a thermostable α-amylase
DIR@IMTECH: CSIR-Institute of Microbial Technology
View Archive Info| Field | Value | |
| Title |
Strain improvement for the production of a thermostable α-amylase
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| Creator |
Sidhu, G.S.
Sharma, P Chakrabarti, T Gupta, J.K. |
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| Subject |
QR Microbiology
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| Description |
Bacillus sp. MK716, a thermophilic and amylolytic soil isolate, produced a thermostable α-amylase active even at 100°C. The enzyme yield was increased 40 times through mutagenesis with ethylmethane sulfonate. The mutated gene was cloned in Escherichia coli-pBR322 system, and the 4.8 kb cloned fragment was mapped with restriction enzymes. Subcloning in Bacillus subtilis of a 2.0 kb portion of the cloned fragment resulted in 107 times increased enzyme yield. The recombinant plasmid was completely stable in B. subtilis.
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| Publisher |
Elsevier Science
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| Date |
1997
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://crdd.osdd.net/open/772/1/tapan1997.pdf
Sidhu, G.S. and Sharma, P and Chakrabarti, T and Gupta, J.K. (1997) Strain improvement for the production of a thermostable α-amylase. Enzyme and Microbial Technology, 21 (7). pp. 525-530. ISSN 01410229 |
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| Relation |
http://dx.doi.org/10.1016/S0141-0229(97)00055-0
http://crdd.osdd.net/open/772/ |
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