Characterization of a fungal amylase from Mucor sp. associated with the marine sponge Spirastrella sp.
DIR@IMTECH: CSIR-Institute of Microbial Technology
View Archive InfoField | Value | |
Title |
Characterization of a fungal amylase from Mucor sp. associated with the marine sponge Spirastrella sp.
|
|
Creator |
Mohapatra, B.R.
Banerjee, U C Bapuji, M |
|
Subject |
QR Microbiology
|
|
Description |
A novel amylase was isolated from the Mucor sp. associated with the marine sponge Spirastrella sp., grown at 30°C. The enzyme has an optimum pH of 5.0 and an optimum temperature of 60°C. The half lives of the partially purified enzyme at 55 and 60°C were 120 and 50 min, respectively. The activation and deactivation energies of the partially purified enzyme were 46.60 and 157.05 kJ mol−1, respectively. The enzyme activity was not affected by the addition of 3% NaCl, 10 mM Ca2+ and 25 mM Mg2+, but was strongly inhibited by EDTA.
|
|
Publisher |
Elsevier Science
|
|
Date |
1998
|
|
Type |
Article
PeerReviewed |
|
Format |
application/pdf
|
|
Identifier |
http://crdd.osdd.net/open/783/1/banerjee1998.pdf
Mohapatra, B.R. and Banerjee, U C and Bapuji, M (1998) Characterization of a fungal amylase from Mucor sp. associated with the marine sponge Spirastrella sp. Journal of Biotechnology, 60 (1-2). pp. 113-117. ISSN 01681656 |
|
Relation |
http://dx.doi.org/10.1016/S0168-1656(97)00197-1
http://crdd.osdd.net/open/783/ |
|