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Production of biosurfactant at mesophilic and thermophilic conditions by a strain of Bacillus subtilis

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Production of biosurfactant at mesophilic and thermophilic conditions by a strain of Bacillus subtilis
 
Creator Makkar, R S
Cameotra, Swaranjit Singh
 
Subject QR Microbiology
 
Description The ability of a Bacillus subtilis strain to grow and produce biosurfactant on different carbon and nitrogen sources under thermophilic conditions (45°C) was studied. The strain was able to reduce surface tension to 34 dynes cm-1 on 2% sucrose, and 32 dynes cm-1 on starch after 96 h of growth. The biosurfactant was stable at 100°C and within
a wide pH range (3.0–11.0). Biosurfactant formation at mesophilic conditions (30°C) was also studied. The organism
was able to produce the maximum amount of biosurfactant when nitrate ions were supplied as the nitrogen source.
The potential application of the biosurfactant in oil recovery from desert oil fields, acidic and alkaline environments is demonstrated. The biosurfactant was identical to surfactin as confirmed by TLC and IR analysis.
 
Publisher Springer Science
 
Date 1998
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/785/1/swaranjit1998.pdf
Makkar, R S and Cameotra, Swaranjit Singh (1998) Production of biosurfactant at mesophilic and thermophilic conditions by a strain of Bacillus subtilis. Journal of Industrial Microbiology and Biotechnology, 20 (1). pp. 48-52. ISSN 1367-5435
 
Relation http://dx.doi.org/10.1038/sj.jim.2900474
http://crdd.osdd.net/open/785/