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Spectral analysis of a nonideal bioreactor for a recombinant fermentation with run-away plasmids

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Spectral analysis of a nonideal bioreactor for a recombinant fermentation with run-away plasmids
 
Creator Patnaik, P R
 
Subject QR Microbiology
 
Description An earlier application of spectral analysis to an isothermal, perfectly mixed, fed-batch fermentation [11] showed that it provides insight into the performance and reveals significant differences between key extra-cellular and intra-cellular (recombinant) variables. To enable application on a practically useful scale, the methodology has been extended to a bioreactor with temperature variation, incomplete mixing of the broth and Gaussian disturbances. For the same system, #-galactosidase produced by a recombinant E. coli strain, there are again differences between the rDNA and its protein, on the one hand, and the concentration and mass fraction of recombinant cells, on the other. For certain combinations of the dilution rate and the degrees of mixing, the bioreactor is only marginally stable, implying that a sufficiently large disturbance can trigger unstable behavior.
 
Publisher Springer Science
 
Date 1999
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/807/1/patnaik1999.2.pdf
Patnaik, P R (1999) Spectral analysis of a nonideal bioreactor for a recombinant fermentation with run-away plasmids. Bioprocess Engineering, 20 (1). p. 31. ISSN 0178515X
 
Relation http://dx.doi.org/10.1007/s004490050556
http://crdd.osdd.net/open/807/